CRL-2888 WPE-int 人正常前列腺上皮細胞

ATCC® Number: CRL-2888™ Price: $329.00

Designations: WPE-int

Depositors: MM Webber, EJ Tokar

Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

CRL-2888 WPE-int 人正常前列腺上皮細胞

Source: Organ: prostate, normal, peripheral zone

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794]

Receptors: androgen receptor, expressed （ [92794] upregulated upon exposure to androgen）

Tumorigenic: NO

Antigen Expression: kallikrein 3 （ KLK3）; prostate specific antigen （PSA）; expressed （upregulated upon exposure to androgen） [PubMed: 16351690] [92794]

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18

Age: 54

Gender: male

Ethnicity: Caucasian

Comments: WPE-int cells were derived from the RWPE-1 cell line （ATCC CRL-11609） after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 （HPV-18） genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line （ATCC CRL-2887）. They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line （ATCC CRL-2887）, which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.

The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. （personal communication from depositor）.

Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen （GIBCO） as part of a kit: Keratinocyte Serum Free Medium （K-SFM）, Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line （bovine pituitary extract （BPE） and human recombinant epidermal growth factor （EGF）. To make the complete growth medium, you will need to add the following components to the base medium:

0.05 mg/ml BPE - provided with the K-SFM kit

5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.

ATCC® Number: CRL-2888™ Price: $329.00

Designations: WPE-int

Depositors: MM Webber, EJ Tokar

Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

Source: Organ: prostate, normal, peripheral zone

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794]

Receptors: androgen receptor, expressed （ [92794] upregulated upon exposure to androgen）

Tumorigenic: NO

Antigen Expression: kallikrein 3 （ KLK3）; prostate specific antigen （PSA）; expressed （upregulated upon exposure to androgen） [PubMed: 16351690] [92794]

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18

Age: 54

Gender: male

Ethnicity: Caucasian

Comments: WPE-int cells were derived from the RWPE-1 cell line （ATCC CRL-11609） after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 （HPV-18） genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line （ATCC CRL-2887）. They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line （ATCC CRL-2887）, which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.

The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. （personal communication from depositor）.

Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen （GIBCO） as part of a kit: Keratinocyte Serum Free Medium （K-SFM）, Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line （bovine pituitary extract （BPE） and human recombinant epidermal growth factor （EGF）. To make the complete growth medium, you will need to add the following components to the base medium:

•0.05 mg/ml BPE - provided with the K-SFM kit

•5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.

CRL-2888 WPE-int